A spokesperson from the Mid North Coast Local Health District has confirmed two men from the Port Macquarie-Hastings area have been diagnosed with COVID-19.
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The news comes after a 14-year-old female student from St Columba Anglican School, who did not travel overseas, contracted the virus.
The Mid North Coast Local Health District spokesperson said one man is aged in his 50s, while the other is aged in his 80s.
Both men have recently returned to the region after travelling overseas and are now in isolation.
The spokesperson said the latest cases are not related to that of the 14-year-old girl, who was first to be diagnosed in Port Macquarie.
The Mid North Coast Local Health District is contacting people who have been close contacts of the men and asking them to self-isolate for 14 days from their last contact.
Read more: NSW braces for 'comprehensive shutdown'
To the north, the Northern NSW Local Health District spanning an area from Grafton to the Queensland border has not recorded any new COVID-19 cases in the past 24 hours while in the south, in the Hunter New England Local Health District, there have been an additional four cases.
Meanwhile, the Minister for Health and Medical Research has signed a public health order to declare Lord Howe Island, east of Port Macqarie, as a public health risk area in order to reduce the risk of COVID-19 importation into the community.
The order commenced at 5am on March 22. It restricts access to Lord Howe Island to residents of Lord Howe Island, health service workers and other essential service workers. The order also directs people on Lord Howe Island into home quarantine for 14 days after arrival - with some exceptions made for essential workers. The order extends to June 18 but may need to be extended further.
As part of the additional measures put in place to monitor cruise ships, NSW Health has identified four separate cruises affected by COVID-19, in addition to the Ruby Princess.
NSW will now undertake a "comprehensive shutdown" of non-essential services as the number of coronavirus cases in the state rises past 500.
More than 52,600 tests have been conducted statewide.
Premier Gladys Berejiklian says supermarkets, petrol stations and pharmacies are among the businesses that will stay open.
Schools will remain open on Monday, although the premier is planning to make further announcements on education.
Visit the NSW Health website for more information.
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